PEG-MGF FAQs

PEG-MGF FAQs

Does PEG-MGF have to be injected into the muscle?
Due to its long half-life, PEG-MGF (Pegylated Mechano Growth Factor, also known as IGF-1ec) does not need to be injected into muscle tissue but given that MGF is normally released in the body as a result of muscle trauma, it is recommended, but not essential, that you inject PEG-MGF after weight training into the muscle group just worked. Since injections are done with an insulin syringe this limits most people to bicep, calf muscle and possibly shoulder injections. Other muscles generally have a layer of subcutaneous fat covering them which will prevent an insulin syringe from reaching the muscle. The only alternative would be to use a syringe barrel + longer syringe (such as 25g) to inject, however dosing would be difficult with this method.
Thankfully, since the addition of PEG (pegylation) to MGF takes its half-life from a few minutes to a few days, an injection into a muscle group which was not just trained, or a sub-q injection, means the peptide will still eventually reach the desired receptors and carry out its positive effects on muscle recovery and growth.

When should I inject IGF-1 LR3 and PEG-MGF and how much?
Due to its long half-life and mechanism of action the injection timing of IGF-1 LR3 is not so critical, however most people inject it pre or post weight training when the muscle receptors are more sensitive. The usual IGF-1 LR3 dosage is 50mcg per day taken on weight training days only. Since PEG-MGF is a growth factor which is naturally only released in response to muscle trauma (such as weight lifting), you should always aim to inject as soon as possible after your workout at a dosage of at least 200mcg per injection.

How long should I inject IGF-1 LR3 and PEG-MGF for?
Both IGF-1 LR3 and PEG-MGF should be used for 4 weeks at a time with the same amount of time off, i.e. one month on, one month off. The reason for this is because the products become less effective at this point due to down-regulation of the receptors (i.e. your muscles are no longer sensitive to the peptides). You therefore may like to take IGF-1 LR3 for one month, then MGF for a month and keep rotating them so you will be using either one of them continuously for maximum results.

Can IGF-1 LR3 and PEG-MGF be taken together?
It is not recommended to inject IGF-1 LR3 and MGF together since they compete for the same receptor sites and IGF-1 LR3 would be a much more powerful binder and probably render the PEG-MGF useless. A better solution would be to rotate them and take IGF-1 LR3 for 4 weeks then PEG-MGF for 4 weeks and keep repeating. If you did want to take both products together the best routine would be to inject IGF-1 LR3 first thing in the morning before breakfast, lift weights in the afternoon and take your PEG-MGF post workout directly into a muscle. It's best to avoid IGF-1 LR3 injections in the evening since it can hinder the body's natural release of growth hormone due to negative feedback on the hypothalamus.

My IGF-1 levels are normal on blood tests; shouldn't they be higher when using IGF-1 LR3?
IGF-1 blood tests look only for bound IGF-1 while the purpose of IGF-1 LR3 is to keep it unbound and therefore bioavailable meaning it will not show up on conventional blood tests. It is the same scenario as for blood tests which examine testosterone levels. Many people can have results showing low or normal testosterone levels, but have high "free testosterone" levels - which is much more important, since only testosterone which is not bound to SHBG (sex hormone binding globulin) can be used by the body.
Therefore your levels of IGF-1 on a blood test are not of much importance and do not take into account the increase from IGF-1 LR3 usage, as they only look at bound IGF-1 (which cannot be used by the body since it's attached to binding proteins).

What are the side effects of IGF-1 LR3 and PEG-MGF?
With PEG-MGF no side effects have been noted by users of the peptide. With IGF-1 LR3 however minor side effects have been mentioned such as hypoglycaemia (low blood sugar), which occurs due to the product being "insulin-like". This can be overcome by consuming carbohydrates post injection to stabilize blood sugar. Some users also notice very strong "pump" effects in the muscle which can make it difficult to do high repetition sets of weights.

How long is IGF-1 LR3 stable after mixing?
If your IGF-1 LR3 peptide vial is freeze-dried, then it will usually be stable for at least 2 months (8 weeks) in the refrigerator after being reconstituted (mixed) with a sterile solution. Since the recommended daily dosage is 50mcg, even if the product is only injected 3-4 times per week this should be plenty of time to use the vial before it could possibly degrade.

What solutions can IGF-1 LR3 be mixed with?
Legitimate manufacturers of IGF-1 LR3 peptides have advised that there is no advantage to specific types of mixing solution over others. The peptide is equally soluble and stable in acetic acide 0.6% solution and bacteriostatic water.

Should IGF-1 LR3 be injected into the muscle or fat?
Due to its long half-life, even if you injected your IGF-1 LR3 peptide into fat, it would still find its way to the binding sites in the muscles of your body. If you have no issue with either type of injection, an intramuscular injection is recommended to allow the start exhibiting its localized effects immediately.

More Peptide Info...

Peptide Combinations Guide

Peptide Combinations Guide

 

Growth Hormone (GH) and IGF-1 are naturally occurring hormones in the human body responsible for many enviable aesthetic traits such as muscle mass, leanness and a firm/even skin tone. As people age, levels of growth hormone rapidly decline and this is one of the main reasons humans put on weight, lose muscle mass and develop sagging/uneven skin. It's no surprise then that synthetic Human Growth Hormone is a sought after product for anti-aging by persons looking to remain youthful, bodybuilders looking to put on muscle mass and people in general who are looking to "tone up" or lose stubborn belly fat.


It is important to note that the products mentioned on this website are not the actual synthetic HGH (although HGH Frag 176-191 is the part of the "real" HGH which contributes to fat loss only). But in many respects these peptide analogues are far superior to the HGH 191aa drug for 3 reasons:
Actual HGH is very difficult or impossible to obtain in most countries and is one of the most faked/counterfeit drugs sold online.


If HGH can be obtained legally from a physician for anti-aging it's extremely expensive, with 10iu (units) costing as much as $200USD. As a comparison, the 10iu equivalent of GH releasing peptides (approximately half of one vial) sells online for approximately $20USD, a full 90% cheaper than actual HGH for the same, if not better, positive effects on the body.


Since actual HGH shuts down the body's natural pituitary gland, when you stop injecting it, your body's own ability to produce Growth Hormone is hindered and you will suffer a rebound of negative side effects such as fat gain, muscle loss and loss of skin tone/elasticity. This means you may end up doing yourself more harm than good. Since GH releasing peptides only stimulate your body's own natural production, there is no rebound negative effects if you stop usage.


The following guide indicates the best way to combine different peptides depending on your experience level of diet/training and also your goal:


Fat Loss
Muscle Building
Anti-Aging


As a general introduction, you should understand the different classes of peptides as this largely determines their combinations:


Growth Hormone Releasing Hormones (GHRH): include Modified GRF 1-29 and CJC-1295 DAC, are peptides which stimulate the pituitary gland to release stores of the body's natural Growth Hormone (GH).


Growth Hormone Releasing Peptides (GHRP): include Ipamorelin, GHRP-2 and GHRP-6, peptides which stimulate the release of a hormone called "Ghrelin" in the stomach, which then in turn causes GH to be released. GHRP's cause a much more significant release of GH than do GHRH, meaning that mg for mg, a peptide like GHRP-6 is three times more potent than Modified GRF 1-29. However, when taken together, they become approximately ten times more potent than either one alone.


IGF-1 Peptides: include IGF-1 LR3 and IGF-1e (also known as MGF or Mechano Growth Factor). IGF-1 is responsible for many of the positive effects of GH on fat loss and muscle building therefore they offer a good addition, especially if your goal is to build muscle, as they are both responsible for creating new muscle cells which can hypertrophy (get bigger) through weight training.


HGH Fragment 176-191: is a peptide which does not stimulate the release of GH but is instead a piece (or "fragment") of the full synthetic 191aa HGH molecule. More importantly, it is the fragment of GH which is responsible for fat loss, meaning it gives all of the fat loss benefits without any side effects.


As a general rule, regardless of your goal, if you are just looking to take one product, with the least amount of fuss and injections as possible, then it should be CJC-1295 DAC at 2mg (1 vial) per week. Due to its long half-life it causes your overall level of GH (Growth Hormone) to rise, and you will therefore see some improvements in things which go along with having higher levels of GH and IGF-1 such as improved body shape, sleep, skin and general wellbeing (although it can make you tired for the first 1-2 weeks while the body adjusts). Your dosage can be taken as just one injection per week (note that you may notice a head rush/flushing for 15-20 minutes after your injection due to the release of GABA in the body, a sign the product is working).
For information about more intricate and advanced usage of GH peptides please read on.


Fat Loss


The most potent weight loss peptide is HGH Fragment 176-191 which is the part of the Growth Hormone molecule responsible for fat burning. In HGH Frag Studies, it has been proven to reduce body fat, particularly in the abdominal area. The second most potent fat loss peptide is CJC-1295 DAC since it causes the overall GH level to rise in the body (the opposite of what happens naturally as a person gets older, which is why people tend to put on weight as they age). If your only goal is fat loss, it's often best to avoid the use of GHRP products (GHRP-6, GHRP-2 or Ipamorelin) since they can stimulate hunger and/or raise cortisol, both of which can be counterproductive to fat burning.


Diet Considerations


For GH to exhibit its fat burning effects, insulin must NOT be present. Insulin release in the body is caused mainly by consuming carbohydrates, although all types of macronutrients (carbs, fat and protein) still cause the release of insulin to some extent. Since HGH Frag works by causing the body to break down and release stored fat for use as energy, if you have recently consumed calories (food or beverage) your body will just use that for energy instead and little extra fat will be burnt. If however there is no food present for the body to use as energy, it will use the stored fat which the HGH Frag has caused to be released and you will notice reductions in body fat over the ensuing weeks.


Due to CJC-1295 DAC's long half-life the timing of meals is not important and this is what makes it an appealing addition to HGH Frag 176-191 to accelerate fat loss. To get the most out of your peptide usage for fat loss, the following guidelines should be followed:


Avoid eating/drinking anything with calories for three (3) hours either side of your injection.
Try to make all your meals throughout the day high protein, low fat and low carbohydrates (eg. meat/fish with vegetables/salad).
Have as few meals as possible during the day as periods of fasting have been shown in many studies to improve fat loss and also longevity (i.e. eating less will make you live longer).


Sample Peptide Cycles


Beginners


HGH Frag 176-191 at 250-500mcg per day.


Advanced


HGH Frag 176-191 at 250-500mcg per day + CJC-1295 DAC at 300mcg per day or;
HGH Frag 176-191 at 250-500mcg per day + Modified GRF 1-29 at 100-300mcg per day (split into dosages of 100mcg)


Example Injection Routines


Example 1 - Night Time Injection (recommended)
Ensure you do not eat or drink anything containing calories within three (3) hours of going to bed (with the exception of water, diet sodas, coffee/tea with artificial sweeteners).
Take your HGH Frag 176-191 injection just before getting into bed and your body will therefore be burning stored fat for the duration of your sleep.
If possible, do some cardio first thing in the morning and wait as long as possible before having breakfast to allow the fat burning to continue throughout the morning/day.


Example 2 - Morning Injection
Wake up and inject your HGH Frag 176-191 (250mcg to 500mcg is a good dosage depending on your budget).
Wait as long as possible before having your first meal (the longer you wait the more fat you will burn).
When you do eat, try to make the meal high protein, low fat and low carbohydrate (example meat and salad/vegetables).
If possible, try to do some cardio in the hours after your injection to increase the fat burning effect.


Note: If you are a person concerned about loss of muscle mass, you can consume a small amount of protein every 2-3 hours (amino acid tablets such as EAA and BCAA are good for this purpose and can be purchased from any health food shop or ordered online). However there is little reason to be concerned about muscle loss because when fat is available for energy, such as following HGH Frag 176-191 injections, protein and therefore muscle mass are spared.


Adding CJC-1295 DAC
You can add CJC-1295 DAC at 2mg once per week (or 300mcg each day along with your HGH Frag 176-191 injections - they can be mixed in the same syringe without any issues). You should take a break from CJC-1295 DAC every few months to give your pituitary gland a rest at which time you can continue to use HGH Frag 176-191 on its own, or you can substitute the CJC-1295 DAC with the short acting Modified GRF 1-29 at 100-300mcg per day (split into injections of 100mcg).


Muscle Building


Growth Hormone (GH) exhibits its muscle building effects mainly after its conversion to IGF-1 (Insulin-Like-Growth Factor). This makes IGF-1 an ideal choice of peptides for muscle building, especially since the IGF-1 LR3 version has an extended half-life which allows it to remain active in the muscles for many hours to complete its muscle building stimulatory effects. Likewise, if injected after a workout, the IGF-1 variant Mechano Growth Factor (also known as MGF or IGF-1e) is known to multiply muscle cells and contribute to muscle development. Furthermore, since IGF-1 is a by-product of GH, any peptide which increases levels of GH in the body such as a GHRP product or CJC-1295 product will obviously lead to increased lean muscle mass.


Diet Considerations
For Growth Hormone (GH) to perform its anabolic (muscle building) affects it requires the presence of the body's most anabolic hormone: insulin. This is in contrast to GH related fat loss which requires insulin to be absent. However, since GHRP and fast-acting GHRH (Growth Hormone Releasing Hormone) products (i.e. Modified GRF 1-29) still need time to stimulate the body to release GH from the pituitary gland, the insulin spike must come after the injection and not before, otherwise the GH release will be blunted.


The only exception to this is of course CJC-1295 DAC since it's long-half life and continual release of GH means it is not affected by food timing.
To get the most out of your peptide usage for muscle building, the following guidelines should be followed:


If injecting just a GHRP or GHRH product on their own, avoid eating/drinking anything high in fat for 3 hours before your injection and anything high in carbohydrates for 2 hours before (i.e. always do your injection on an empty stomach), otherwise the amount of GH release they cause may be significantly blunted leading to poor results.
If injecting both a GHRP and GHRH together (e.g. 100mcg of both GHRP-6 and Modified GRF 1-29) studies have proven that their ability to release GH returns to full-strength as little as 1 hour (60 minutes) post-meal. This gives users greater flexibility with their meal timings, especially since consuming sufficient calories is so critical to building muscle.
Whether injecting GH peptides alone or along with others, always wait at least 20 minutes after your injection before consuming anything. Once at least 20 minutes has passed, consume a food/beverage high in protein and/or carbohydrates to stimulate an insulin spike (if you inject in the morning and around your workout, this meal/shake should be high protein and high carbohydrates, if you inject at night this consumption should be protein only as protein is sufficient enough to spike insulin, but without the negative impact on fat gain which carbohydrates can contribute to).


Sample Peptide Cycles


Beginners


CJC-1295 DAC at 2mg per week or;
GHRP Product (GHRP-6, GHRP-2 or Ipamorelin) at 200mcg once per day.


Intermediate


GHRP Product at 200mcg + Mod. GRF 1-29 at 100mcg (2 times per day) or;
GHRP Product at 200mcg + CJC-1295 DAC at 100mcg per day (2 times per day).


Advanced


GHRP Product at 200mcg + CJC-1295 DAC at 100mcg (2 times per day) + IGF-1 at 50mcg after workouts or;
GHRP Product at 200mcg + Mod. GRF 1-29 at 100mcg (2 times per day) + PEG-MGF at 200mcg after workouts.


Example Injection Routines


Beginners


CJC-1295 DAC
2mg taken once per week, at any time of day.


GHRP + GHRH (once per day)
Inject your dosage (ensuring you have not consumed any food/beverages for at least 1 hour before, an optimal time would be first thing in the morning).
Ingest a protein only or protein and carbohydrate meal afterward to create an insulin spike.
Do weight training in the hours afterwards.


Intermediate


GHRP + GHRH (twice per day)
Inject your GHRP + GHRH peptides together in the same syringe (ensuring you have not consumed any food/beverages for at least 1 hour before, an optimal time would be first thing in the morning).
Ingest a protein only or protein and carbohydrate meal afterward to create an insulin spike.
Do weight training in the hours afterwards.
at least 1 hour after your dinner (or last meal of the day), take your second GHRP + GHRH injection.
If you are trying to control your body fat then have a protein only meal 20-30 minutes afterwards, otherwise a protein/carbohydrate meal will create a better insulin spike.


Advanced


GHRP + GHRH + IGF-1
Follow the same routine as shown above for "intermediate" persons. However, as soon as possible after your weight training you should also inject 200mcg of PEG-MGF (IGF-1e) and/or 50mcg of IGF-1 LR3 preferably into a muscle (although due to the long half-life of both products, sub-q injections are also acceptable). If injecting intramuscularly, you should make sure that the muscle you are injecting into is not covered by a thick layer of fat. Usually due to the length of insulin syringe needles, injections are therefore limited to the biceps for most persons.


While GHRP + GHRH can be injected each day with great benefit, even if you don't do weight training on that day. On the other hand, IGF-1 LR3 and MGF injections should be reserved for post-workout only.


Anti-Aging


For the Anti-Aging crowd, we recommend choosing only 1 peptide, rather than a combination. The reason for this is that as you get older your GH levels decline rapidly and therefore you will benefit from any kind of GH increase meaning there is no need to overdo things with multiple peptides. If you wish to use more than one peptide, we recommend cycling a GHRP product (GHRP-6, GHRP-2 or Ipamorelin) every 3-6 months with CJC-1295 DAC for two reasons.


The first reason is that CJC-1295 DAC is a GHRH (growth hormone releasing hormone) acting directly at the pituitary, while GHRP products indirectly stimulate GH by causing the release of Ghrelin. Rotating the products would therefore ensure one method of GH stimulation does not get "worn out" from repeated exposure to the peptides. The second reason is that even though CJC-1295 DAC has been proven safe in much higher dosages than we recommend, since it causes a continual GH release (GH bleed) no one can be certain how continual use would affect the pituitary in the long-term, so it's a case of being "better safe than sorry" and never using it for longer than 6 months at a time without a break.


Diet Considerations


For CJC-1295 DAC there are no particular diet restrictions that need to be followed due to its long half-life. For GHRP products the following should be observed as insulin and fatty acids can blunt the release of GH in the body and therefore make your injections less effective:
Avoid eating/drinking anything high in fat for 3 hours before your injection and anything high in carbohydrates for 1-2 hours (always do your injection on an empty stomach).
Wait at least 20 minutes after your injection before eating/drinking anything with calories.


Injection Amounts


CJC-1295 DAC taken at 2000mcg (2mg) once per week or;
GHRP Product (GHRP-2, GHRP-6 or Ipamorelin) taken at 200mcg per day.


Example Injection Routines


CJC-1295 DAC
2mg taken once per week, at any time of day.


GHRP-2, GHRP-6 or Ipamorelin


Example 1 - Night Time Injection (recommended to reduce possible tiredness during day).
Ensure you have an empty stomach (i.e. 2-3 hours since your last meal).
Inject your GHRP peptide and go straight to bed.


Example 2 - Morning injection
Take your injection of the GHRP product first thing in the morning at 100mcg.
Wait at least 20 minutes before having breakfast or any beverages (including coffee/tea).
(All info gathered via internet)

More Peptide Info...

EXPERIENCES WITH IGF-LR3

IGF1-LR3 What is it?
IGF-1 is basically a polypeptide hormone that has the same some of the same molecular properties as insulin. IGF dose actually stand for insulin-like growth factor. IGF-1 is mainly responsible for long bone growth in children and it also affects muscle growth and repair of adults. Long R3 IGF-1 is a more potent version of IGF-1. It's chemically altered i like to think "enhanced" to prevent deactivation by IGF-1 binding proteins in the bloodstream. This results in a longer half-life of 20-30 hours instead of 20 min... So that means a far more effective version than the short chain we re perhaps more familiar with.

IGF1-LR3 What does it do?

IGF-1LR3 greatly boosts muscle mass by inducing a state of muscle hyperplasia (increase in number of new muscle cells) in the MUSCLE WHERE ITS INJECTED!!
So think of it as muscle cell proliferation, or even the splitting of the cell so 1 becomes 2... Thats why its perfect on cycle when you get increased muscle cell growth too.
But why is IGF better than HGH? The reason being is HGH causes IGF levels to rise in the liver first, then then the muscle, Whereas IGF-LR3 causes localized IGF levels to rocket.

What other benefits?

Taken from a study in Germany..

"Tissue build up is one of the main features of IGF-1, so I'd say it's of greater value. IGF-1 can genetically change muscular and cellular counts within the body; it can also enhance the body's ability to regenerate damaged tissue. In fact, IGF-1 is now under intensive research for its potential to repair tissue in burn patients, and for its regenerative effects on AIDS patients suffering from muscular wasting. Immediate effects are, of course, impossible to observe since it takes a respectable amount of time to see any visible changes in muscular repair"
But muscle size and shape can be seen quite quickly through a course of IGF-LR3




Dosage and use..

The best dose for muscle cell proliferation is 40mcg bi-laterally for men, and 20mcg for women... so 40mcg in one bicep and 40mcg in the other Pre-workout.
And 20 in each bicep for women. This can be done in any 2 matching muscle groups.
This should be done for 40 days max and then have 30 days off.
What you need to be careful of is adequate carb intake when using IGF, Especially this version as its long lasting, it will literally leech glucose to cause its localized muscle enhancing effects, similar to the way in which insulin works, This goes on for 7-10 hours, You should take in 20g of carbs 5 grams slow release and 15 g fast, for every hour its active for the first 7 hours.. so it looks like this.

Based on a mans dose, so a women half this.

Pre-workout.. 40mcg bi-laterally. Then pwo meal of 40g carbs, 30 slow release and 10g fast release carbs... that covers you for 2 hours.

Post-workout. 40 grams carbs 30 grams low gi or slow release, and 10g fast release carbs. That covers you for 2 more hours..

2 Hours later so 4 hours since the injection the same again... that takes you up to 6 hours, at this point just consume carbs when you feel the need... if you feel light headed, or any signs of hypoglycemia.


Some people prefer to dose 70 g carbs with there pre-workout meal, and 70 grams of carbs 4 hours later, but the most anabolic option is the one i outlined, causing a constant supply of everything you need to maximize its effects.

Only dose on workout days and a maximum of 3 times a week.

Use a 1" insulin syringe and only inject directly into muscle, and reconstitute with Acetic acid, once reconstituted keep refrigerated and use within 6 months.

Why dose pre-workout?

When do you get muscle cell proliferation? This is when the muscle actually breaks down during intense exercise, this causes localized IGF-1 levels to sky rocket, Thats why Arachadionic acid works, because it increased localized IGF-1 levels, causing an increased inflammatory response to muscle degradation or breakdown, So by using IGF-LR3 pre workout you receive the benefits of Hyperplasia straight away, without waiting for nearly 2 hours to use your IGF.. So you increase the window of growth time period, during its most significant period.. You have just provided your muscle everything it needs for muscle growth at EXACTLY the right time.. before its broken down. Plus by eating your Pre workout meal you fuel your workout and allow for your muscles to take advantage of the huge pumps IGF-LR3 can cause as your muscles are flooded with nutrients, blood and increased localized IGF-1 levels.

Russians experiences.

Using the protocol i outlined above, after weeks of experimenting and trying new ideas i found great success.
I focused on my triceps and biceps, In 4 weeks of the above protocol i added a depth to my tricep horseshoe and shape that i haven't seen in many other bodybuilders, and i increased the severity of my bicep peak, 0.5" increase in arm size, now that may not seem much, but when you think on most anabolic cycles that last 5 weeks or more those kind of gains are not really that common, i saw that as a big result especially as i ran it stand alone, With no additional anabolics.

The next time i run it, i will incorporate Need2slin to increase liver igf-1 levels and increased insulin response so increasing the effectiveness of the igf-lr3.


Possible sides.

Lethargy is a big one as carbs are used to increase muscle cell proliferation and not used as readily for fuel.
The big one to watch out for is burning from the acetic acid when its reconstituted, and the Hypoglycemia, Always have plenty of carbs on hand in case the sides hit you hard!!!


Enjoy your use of IGF-LR3, add some shape and size to lagging parts.

Kind regards..

RS

More Peptide Info...

Beginner’s Guide To IGF1-lr3

Beginner’s Guide To IGF1-lr3

• IGF-1 Reconstitution
• Making 0.6% Acetic Acid from Vinegar
• Injection Technique
• Sterile Procedure
• Items You Will Need
• …and more!

Author: PapaPumpSD
Date: 05/31/2008
Version: 1.0

Special thanks to Bobaslaw
for reviewing prior to release


Table of Contents

PREFACE 2
IGF-1LR3 OVERIVEW 3
0.6% ACETIC ACID OVERVIEW 4
MAKING 0.6% ACETIC ACID 4
RECONSTITUTING IGF-1LR3 6
INJECTING IGF-1LR3 6
STERILITY 7
PRE-INJECTION ASPIRATION 8
INJECTION PROCEDURE 8
Back-Loading With Bacteriostatic Water (BW) 8
Items you will need 9
Injection Directions 9
GLOSSARY 10



Preface
The goal of this guide is to help both those that have not used IGF-1lr3 before and for those that simply would like a methodical approach to the “mechanics” of running it. This guide does not expand on the biochemistry of IGF-1, aside from a very simple introduction to it. I suggest reading a book or searching forums to educate yourself about the biochemistry of “peptides” or “IGF” if you require in-depth knowledge.

I am not a physician, thus cannot and do not diagnose ailments or diseases and/or nor do I suggest that IGF-1 is a remedy for any illness or diseases. IGF-1 should be treated with much respect. It is research compound, thus you should use at your own risk.

Currently (05/31/2008), in the United States, IGF-1lr3 is a research compound. It is legal to own this substance to the best of my knowledge (at current time). I am not an attorney, so please review your local law(s) regarding possession and administration of this therapeutic protein.

I do not condone the usage of IGF-1lr3 unless you are qualified to do so. This guide is provided as a research & development tool only.

IGF-1lr3 Overivew

Background:
Long Arg3 Insulin-like Growth Factor-I (Long-R3-IGF-I) is an 83 amino acid analog of IGF-I comprising the complete IGF-I sequence with the substitution of an Arg for the Glu at position 3 (hence R3), and a 13 amino acid extension peptide at the N-terminus. Long-R3-IGF-I is significantly more potent than IGF-I in vitro. The enhanced potency is due to the markedly decreased binding of Long-R3-IGF-I to IGF binding proteins which normally inhibit the biological actions of IGFs.



Description:
Recombinant Human Long-R3-IGF-I produced in E. coli is a single, non-glycosylated, polypeptide chain containing 83 amino acids and having a molecular mass of 9111 Dalton.



0.6% Acetic Acid Overview
Acetic Acid (AA) will be used to reconstitute (turn your lyophilized IGF-1 into a liquid form) your IGF-1. The standard is to use 0.6% AA. This concentration is typically not available for you to purchase. You can make your own 0.6% AA and I will show you how below (many have used this method successfully).

Making 0.6% Acetic Acid
You will have to purchase a few items upfront. Here is a “grocery list” of items you will need. I have provided check boxes for you to check off once you have purchased these items.

Items Needed:
• Distilled white vinegar (grocery store)
• Distilled water (grocery store)
• 0.2-0.22um sterile Whatman syringe filter
• 10mL syringe with a luer lock tip
• ~20-22 gauge needles (just the needles)
• Sterile glass vial (10-20mL)
• Alcohol prep pads – sterile kind (70% isopropyl alcohol)



Quick Guide:
1. Swab the top of your sterile vial with alcohol prep pad (70% isopropyl alcohol)
2. Mix 7.5mL distilled water with 1.0mL vinegar
3. Add Whatman syringe filter
4. Add sterile ~20ga. needle to end of Whatman filter
5. Inject the 8.5mL of solution into the sterile vial
6. You now have sterile 0.6% acetic acid



Detailed Directions:
1. Wash you hands thoroughly
2. Optional: wear alcohol treated exam gloves (rub your gloved hands together with 70% isopropyl alcohol on them until dry)
3. Using a sterile alcohol prep pad, swab the top of your sterile glass vial (into which the acetic acid solution will be held in)
4. Using the 10mL syringe with a ~20ga. needle on the end, draw up 7.5mL distilled water
5. Using the same syringe, now draw up 1mL vinegar
6. Remove needle from the syringe and discard
7. Attach 0.2-0.22um Whatman sterile syringe filter (do not touch the free end that will have a needle on it)
8. Put a new, sterile needle (~20 gauge) onto the free end of the Whatman filter (do not touch needle)
a. Do not use the same needle on the Whatman that was used to originally draw up the unsterile vinegar and distilled water.
9. Put a ~20 gauge sterile needle into the top of your sterile glass vial to act as a vent
10. Inject the acetic acid solution into the vial
11. You are now done and should have sterile 0.6% acetic acid

Notes:
1. These items MUST be sterile: 20-22ga. Needles, whatman filter, glass vial
2. Whatman filter: These small, sterile filters are used to filter the acetic acid solution so it is sterile. It does not matter that the liquid in your syringe (distilled water & vinegar) is not sterile, nor does it matter that the syringe itself is not sterile. Once the liquid goes through the filter it is STERILE. Thus, everything after the filter must be sterile!
3. You will most likely use 1mL (milliliter) of 0.6% AA to reconstitute your IGF-1. Thus, you should make at least 1.5mL. In reality, it’s just as easy to make 8.5mL as I have stated in the above directions. You will have plenty for use later then.
4. Do NOT reuse the Whatman filter nor any needles! Discard immediately.


Reconstituting IGF-1lr3
Reconstitution is simply the addition of the 0.6% AA to your lyophilized IGF-1.
Assumption: 1mg/mL IGF-1/AA (1mg IGF-1 will be combined with 1mL AA; 1mg IGF-1 is the same as 1,000mcg)

1. Swab the top of your IGF-1 vial with a sterile alcohol prep pad
2. Swab the top of your 0.6% AA vial with a sterile alcohol prep pad
3. Using either multiple insulin syringe volumes (example: 2 x 0.5cc) or a single large syringe, obtain 1.0mL of 0.6% AA.
4. In the IGF-1 vial, insert a sterile ~20 ga. needle to act as a vent
5. Inject the 1.0mL of AA very slowly and dribble it down the side of the vial.
a. Be very careful with this peptide as it is very delicate!
6. Remove the needle & syringe and discard
7. Gently swirl the vial or roll between your hands.
a. Again, be very gentle here
8. You now have 1mg/mL of IGF-1
a. This is the same as: 1,000mcg/mL

Notes:
1. If you added 2mL of AA, it would be a 0.5mg/mL
2. I have an Excel calculator that will help you with these calculation.  Found here


Injecting IGF-1lr3
If this is your first time with injections, don’t worry. You will be using a very fine gauge insulin syringe which means you will most likely have nearly effortless injections. These things are so tiny and sharp you may not even feel it penetrating. If you use sterile procedure, aspirate prior to injection, and have diluted your IGF-1/AA solution with enough bacteriostatic water (BW), you should have no issues with your injections and very minimal post-injection discomfort (if any at all!).

I cannot stress enough the importance on two topics: A) sterility, and B) pre-injection aspiration. Always swab the injection site(s) with a sterile isopropyl alcohol (IPA) pad and aspirate prior to injecting the IGF-1. No questions asked!

You will most likely intramuscular (IM) injections, but subcutaneous (sub-q) injections are also followed by some, but current theory is that IM will yield a localized effect. By “localized effect”, I am referring to the effect IGF-1 will have at the injection site. So if you inject IM into biceps, it is thought that your bicep muscles will get more of a dose of IGF-1 than other parts of your body (some which you don’t want to be effected, such as the intestines). Both types of injections will have systemic effects (affecting the body as a whole). Long R3 IGF-1 has an estimated half-life of 20-30hrs (taken from IGTROPIN data).

This guide assumes you will be doing bilateral IM injections. More below.

Bilateral injections are injections that are evenly divided between two muscles. If you are injecting 40mcg (micrograms) bilaterally, you will be injecting 20mcg into the right bicep and 20mcg into the left bicep.

Current theorized best practice is to you inject your peptide post workout (PWO). You have a small window of optimal opportunity. Ideally, you would inject immediately PWO, but some do not like the idea of injecting in a public location, such as the gym. Your next best option is to make your way home ASAP and have your needles loaded and ready (with your alcohol swabs sitting near by).


Sterility
Without a doubt, sterility is a major concern with injections. You have to be conscious of bacteria and other infectious agents at all times when performing injections or other procedures that require sterility (such as reconstitutions and making 0.6% AA).

Bacteria (and viruses, and spores, etc) are invisible to the naked eye. Yet they are everywhere. It is very important that you acquire sterile alcohol prep pads (make sure it says “sterile” before you buy them). They are extremely cheap and effective.

Wash your hands! Before attempting anything requiring sterile technique, wash your hands and dry them with a clean paper towel (not the dirty towel hanging in the bathroom!). For optimal sterility, you may purchase exam gloves (latex or non-latex) and, after putting them on, you can dump some isopropyl alcohol (IPA) onto them and rub your hands together thoroughly. Now you really have sterile hands. Exam gloves are very inexpensive as is the bottle of IPA. IPA can be purchased for ~$1/bottle in the grocery store where the band-aids and whatnot.

I recommend you use a fresh syringe for each injection. Yes, some choose to use one syringe, but my feeling is that the syringes are so inexpensive and the risk of cross-contamination from one injection site to the other isn’t worth the risk. Furthermore, every time your syringe needle has to penetrate something (rubber stoppers in vials, skin, etc) it dulls the tip. Thus, maximum comfort is also achieved with fresh syringes.

This topic of “one or two syringes” can be argued, but if it’s your first time, play it safe and get off to a great start by using 2!

Pre-injection Aspiration
Pre-injection aspiration is what you do after the needle has penetrated the muscle. You must gently and slightly pull back on the needle’s plunger to see if you have hit a vein/artery.

Either of two things will happen upon aspiration: A) bubbles/air and/or clear liquid will appear in the syringe (this is good), or B) blood will appear (bad).

If A) occurs, proceed with your injection. If B) occurs, then simply withdraw the needle, and re-pin a different location in that same muscle. You do NOT want to inject your solution into a vein/artery! This may result in very serious consequences. Don’t worry, you can avoid this by simply aspirating slightly. Have faith in yourself.

Injection Procedure
First, do not get all worked up over injecting IGF-1. Easier said than done, I know. But the reality is, the insulin syringes are extremely gentle. Also, millions of people around the world, including women and children, use these syringes daily to treat Diabetes. So you know it can’t be that bad (seriously)! I highly recommend watching a couple videos on youtube regarding intramuscular (IM) injections to get a general idea of how they’re done if you’ve never witnessed them!

Back-Loading With Bacteriostatic Water (BW)
Back-loading is a process in which you dilute the IGF-1/AA solution that is in your syringe. The point is to dilute the acidity to a point that it will no longer cause tissue necrosis (death/damage) or pain upon injection. It is recommended to dilute no less than 4:1 (4 parts BW to 1 part IGF-1/AA).

Example: If you are injecting 40mcg bilat, IM, you will have two syringes each with 20mcg IGF-1. Assume you want to draw 2 IU IGF-1. You will draw 2 IUs of the IGF-1/AA solution, then draw 2x4 = 8 IUs of BW (four times the amount of IGF-1/AA solution). The total number of IUs in each syringe will be 2 + 8 = 10 IUs. It will not hurt you if you decide to back-load with more BW. It is a personal preference.

***Use my Excel-based “IGF-1” calculator to determine how many IUs you will need for a particular insulin syringe (1cc, 0.5cc, 0.3cc).

Recommended Best Injection Method: Injecting bilaterally, post workout, intramuscularly (Bilat, PWO, IM)



Items you will need
1. Alcohol prep pads
2. 2 insulin syringes
3. Bacteriostatic water (BW)
4. Optional: exam gloves
5. Optional: IPA (to rub gloves with and to clean the surrounding area)

Injection Directions
1. Wash your hands thoroughly
2. Optional: put on exam gloves and rub with IPA until dry
3. Using an alcohol swab, clean the tops of both the IGF-1 vial and the BW vial.
4. Using a fresh alcohol swab, thoroughly clean the injection sites (let dry)
5. Fill each syringe with the appropriate amount of IGF-1/AA solution
a. Do NOT touch the needles to anything but sterile surfaces!
b. It is recommended that you clean/sanitize the area/surfaces you’re working in, in case you mindlessly touch a needle to a table (or other area).
6. Back-loading: Draw up the necessary amount of BW into each syringe.
a. Tilt the needle up and down so the bubble(s) rise and fall, which mixes the solution slightly
7. With the needle pointing up, flick the syringe body to get the bubbles to rise to the needle
8. Slowly expel the air; be careful to not quirt liquid out as this wastes IGF-1
a. It takes >3mL of air to cause harm; small volumes of accidentally injected air will most likely be absorbed by muscle tissue
9. Insert syringe and aspirate by slightly pulling up on the plunger to see if you have hit a vessel. If you see blood, remove needle, and try again (no need to change syringes). If you do NOT see blood, proceed to inject.
10. Perform “7.” thru “9” above on other side.
11. Discard sharps in appropriate container



Glossary

Acetic Acid (AA): An acid that, when diluted to 0.6%, will act as a preservative for your IGF-1. An off-the-shelf version of 5% AA is distilled white vinegar; your IGF-1 may be supplied in acetic acid (usually 0.6%)

Aspiration: The technique of checking to see if your inserted needle is in a blood vessel. It is performed by gently pulling up on the syringe plunger until you either see bubbles/air/clear liquid, or blood. If you see blood, remove needle, and re-try the insertion.

Back-loading: The process of diluting your IGF-1/AA with bacteriostatic water, prior to injection. The purpose is to dilute the acidity of the AA so it doesn’t cause tissue damage and so it doesn’t cause injection burn/discomfort.
A. Draw desired amount of IGF-1/AA solution
B. Back-load with BW: draw desired amount of BW

Bacteriostatic Water (BW): This is water for injection (sterile) that has benzoyl alcohol (BA) added to it to ward of contamination. You use BW to dilute your IGF-1/AA solution prior to injection (aka, “back-loading”).

Bilateral Injection (bilat): An injection which involves the administration of IGF-1 in equal amounts to each side of the body. If you are injecting 40mcg IGF-1 into the biceps bilaterally, you will be injecting 20mcg into each bicep (left & right side).

Distilled Water: Has virtually all of its impurities removed through distillation. Distillation involves boiling the water and then condensing the steam into a clean cup, leaving nearly all of the solid contaminants behind. This is NOT sterile water. It can be purchased in any grocery store in the “water” isle.

Endogenous: Substances that originate from within an organism, tissue, or cell. It is the opposite of exogenous

Exogenous: Refers to an action or object coming from outside a system. It is the opposite of endogenous.

IM: Intramuscular; typically refers to the type of injection where you inject a substance directly into muscle tissue

IGF-1 lr3: A peptide that is responsible for new muscle tissue development; it is synthetic and has a much longer circulatory life than endogenous IGF-1

Lyophilized: The form in which IGF-1 is typically supplied; this is a freeze-dried protein which is performed in a vacuum; appearance may range from a fine, loose white powder, to a white solid “paste”-type substance

PWO: Post Work Out; refers to the time period when the administration of IGF-1 is thought to be the most effective (immediately PWO).

Reconstitution: The addition of 0.6% acetic acid to lyophilized IGF-1r3 to get it into solution. Typically one reconstitutes using 1mL or 2mL of acetic acid, yielding 1mg/mL or 2mg/mL of IGF-1/AA.

Sub-q: Subcutaneous; typically refers to the type of injection where you inject a substance under the skin; this results in systemic distribution of substances

More Peptide Info...

IGF-1 Usage

IGF-1 Usage

IGF stands for insulin-like growth factor. It is a natural substance that is produced in the human body and is at its highest natural levels during puberty. During puberty IGF is the most responsible for the natural muscle growth that occurs during these few years. There are many different things that IGF does in the human body; I will only mention the points that would be important for physical enhancement. Among the effects the most positive are increased amino acid transport to cells, increased glucose transport, increased protein synthesis, decreased protein degradation, and increased RNA synthesis.

When IGF is active it behaves differently in different types of tissues. In muscle cells proteins and associated cell components are stimulated. Protein synthesis is increased along with amino acid absorption. As a source of energy, IGF mobilizes fat for use as energy in adipose tissue. In lean tissue,

IGF prevents insulin from transporting glucose across cell membranes. As a result the cells have to switch to burning off fat as a source of energy.

IGF also mimic's insulin in the human body. It makes muscles more sensitive to insulin's effects, so if you are a person that currently uses insulin you can lower your dosage by a decent margin to achieve the same effects, and as mentioned IGF will keep the insulin from making you fat.

Perhaps the most interesting and potent effect IGF has on the human body is its ability to cause hyperplasia, which is an actual splitting of cells. Hypertrophy is what occurs during weight training and steroid use, it is simply an increase in the size of muscle cells. See, after puberty you have a set number of muscle cells, and all you are able to do is increase the size of these muscle cells, you don't actually gain more. But, with IGF use you are able to cause this hyperplasia which actually increases the number of muscle cells present in the tissue, and through weight training and steroid usage you are able to mature these new cells, in other words make them grow and become stronger. So in a way IGF can actually change your genetic capabilities in terms of muscle tissue and cell count. IGF proliferates and differentiates the number of types of cells present. At a genetic level it has the potential to alter an individuals capacity to build superior muscle density and size.

There is a lot of talk about the similarity between IGF and growth hormone. The most often asked question is simply which is more effective. GH doesn't directly cause your muscles to grow, it works very indirectly by increasing protein synthesis capabilities, increasing the amount of insulin a person can use effectively, and increasing the amount of anabolic steroids a person can use effectively. GH also indirectly causes muscle growth by stimulating the release of IGF when it (the GH) is destroyed in the human body. So one way you could look at it as GH being a precursor to IGF. So to put it simple IGF is more effective at directly causing muscle growth and density increases. IGF is also much more cost effective.

IGF can also be effectively used by itself and gains will still be easily noticeable. With growth hormone you need to use high amounts of anabolics and often insulin to see any gains at all, this is not the case with IGF. IGF can be used by itself and is often used by bodybuilders who bridge between cycles, during this bridge is a good time to use IGF since it has no effect on natural testosterone production so it will therefore allow you to return to normal in terms of hormone levels. A stack of IGF, PGF2a, HCG, and clomid would be a good bridge stack and would allow your body to return to normal and still allow you to retain and make new gains.

IGF is a research drug, it hasn't been approved by the FDA for use as a pharmaceutical and it is currently being researched for nerve tissue repair, possible burn victims, and also as a possible aid in muscle wasting for AIDS patients. There are many different analogs of IGF available, instead of mentioning them all, I will simply mention the two most common and the most effective. Regular recombinant IGF is one of the two, it is also the more expensive and the least effective. Regular IGF only has a half-life of about 10-20 minutes in the human body and is quickly destroyed, it can be combined with certain binding proteins to extend the half-life, but it is not a very simple procedure and there is a more effective and less expensive version available. The most effective form of IGF is Long R3 IGF-1, it has been chemically altered and has had amino acid changes which cause it to avoid binding to proteins in the human body and allow it to have a much longer half life, around 20-30 hours. "Long R3 IGF-1 is an 83 amino acid analog of IGF-1 comprising the complete human IGF-1 sequence with the substitution of an Arg(R) for the Glu(E) at position three, hence R3, and a 13 amino acid extension peptide at the N terminus. This analog of IGF-1 has been produced with the purpose of increasing the biological activity of the IGF peptide."

"Long R3 IGF-1 is significantly more potent than IGF-1. The enhanced potency is due to the decreased binding of Long R3 IGF-1 to all known IGF binding proteins. These binding proteins normally inhibit the biological actions of IGF's."

It is also not as expensive since a media grade version is available which is sufficient for bodybuilding use. There is also a receptor grade available but it is VERY expensive and the only noticeable difference between the two would only be able to be noticed in a laboratory setting. The price on the black market for Long R3 IGF-1 can be seen anywhere from $200-$500 per milligram depending on the source.

The most effective length for a cycle of IGF is 50 days on and 20-40 days off. The most controversy surrounding Long R3 IGF-1 is the effective dosage. The most used dosages range between 20mcg/day to 120+mcg/day. IGF is only available by the milligram, one mg will give you a 50 day cycle at 20mcg/day, 2mg will give you a 50 day cycle at 40mcg/day, 3mg will give you a 50 day cycle at 60mcg/day, 4mg will give you a 50 day cycle at 80mcg/day and so on. The dosage issue mainly revolves around how much money you have to spend, plenty of people use the minimum dosage of 20mcg/day and are happy with the results, and in fact several top bodybuilders use the 20mcg/day dosage and are pleased with the results. IGF is most effective when administered subcutaneously and injected once or twice daily at your current dosage. The best time for injections is either in the morning and/or immediately after weight training.

Another frequently asked question of IGF refers to the real world results, in terms of pure weight gain don't expect to gain 5 lbs. a week like you may off of anadrol or a similar steroid. The only weight you will gain from IGF use is pure lean muscle tissue, with steroids most of the weight gained is water weight. With an effective dosage you can expect to gain 1-2 lbs of new lean muscle tissue every 2-3 weeks and these effects can be increased with the use of testosterone, anabolic steroids, and insulin use. Increased vascularity is also very common, people report seeing veins appear where they never have before. And yet another effect reported is the ability to stay lean while bulking with heavy dosages of steroids and TONS of food while on an IGF cycle, this is perhaps the most pleasing effect. Increased pumps are also noticeable almost immediately, the pumps can almost become painful, pumps are even noticeable when doing cardio.

Overall, IGF is a very exciting drug due to its ability to alter ones genetic capabilities. If you can find a trustworthy source and you use it correctly it can be a VERY useful tool in your bodybuilding drug arsenal.

More Peptide Info...

Understanding IGF-1

Understanding IGF-1

To understand how IGF-1 works you have to understand how muscles grow. The ability of muscle tissue to constantly regenerate in response to activity makes it unique. It’s ability to respond to physical/mechanical stimuli depends greatly on what are called satellite cells. Satellite cells are muscle precursor cells. You might think of them as "pro-muscle" cells. They are cells that reside on and around muscle cells. These cells sit dormant until called upon by growth factors such as IGF-1. Once this happens these cells divide and genetically change into cells that have nuclei identical to those of muscle cells. These new satellite cells with muscle nuclei are critical if not mandatory to muscle growth.

Without the ability to increase the number of nuclei, a muscle cell will not grow larger and its ability to repair itself is limited. The explanation for this is quite simple. The nucleus of the cell is where all of the blue prints for new muscle come from. The larger the muscle, the more nuclei you need to maintain it. In fact there is a "nuclear to volume" ratio that cannot be overridden.
Whenever a muscle grows in response to functional overload there is a positive correlation between the increase in the number of myonuclei and the increase in fiber cross sectional area (CSA). When satellite cells are prohibited from donating new nuclei, overloaded muscle will not grow (Rosenblatt,1992 & 1994; Phelan,1997). So you see, one important key to unnatural muscle growth is the activation of satellite cells by growth factors such as IGF-1.

IGF-1 stimulates both proliferation (an increase in cell number) and differentiation (a conversion to muscle specific nuclei) in an autocrine-paracrine manner, although it induces differentiation to a much greater degree. This is in agreement with the Dual Effector theory. In fact, you can inject a muscle with IGF-1 and it will grow! Studies have shown that , when injected locally, IGF-1 increases satellite cell activity, muscle DNA content, muscle protein content, muscle weight and muscle cross sectional area (Adams,1998).

On the very cutting edge of research scientists are now discovering the signaling pathway by which mechanical stimulation and IGF-1 activity leads to all of the above changes in satellite cells, muscle DNA content, muscle protein content, muscle weight and muscle cross sectional area just outlined above. This research is stemming from studies done to explain cardiac hypertrophy. It involves a muscle enzyme called calcineurin which is a phosphatase enzyme activated by high intracellular calcium ion concentrations (Dunn, 1999).
Note that overloaded muscle is characterized by chronically elevated intracellular calcium ion concentrations. Other recent research has demonstrated that IGF-1 increases intracellular calcium ion concentrations leading to the activation of the signaling pathway, and subsequent muscle fiber hypertrophy (Semsarian, 1999; Musaro, 1999).
I am by no means a geneticist so I hesitated even bringing this new research up. In summary the researchers involved in these studies have explained it this way, IGF-1 as well as activated calcineurin, induces expression of the transcription factor GATA-2, which accumulates in a subset of myocyte nuclei, where it associates with calcineurin and a specific dephosphorylated isoform of the transcription factor nuclear factor of activated T cells or NF-ATc1.
Thus, IGF-1 induces calcineurin-mediated signaling and activation of GATA-2, a marker of skeletal muscle hypertrophy, which cooperates with selected NF-ATc isoforms to activate gene expression programs leading to increased contractile protein synthesis and muscle hypertrophy. Did you get all that?

More Peptide Info...

When to Take IGF-1 to Maximize Binding

I’ve read that serum (blood) binding proteins, mainly IGF-bp3 (insulin-like growth factor, binding protein-3) with the acid labile unit, act as carriers to greatly increase the half-life of the IGF-1 molecule in the blood.

 I know that if injected subcutaneous, IGF-1 is cleared from the blood in minutes. If bound to binding proteins, IGF-1 can freely dissociate, acting as a reservoir when needed. I do understand completely that only free drug can interact with receptors, but what is, in your expert opinion, the best form of IGF-1 to take?

     The problem is, how do you we know how much IGF-1 (if any) will dissociate from the proposed binding protein (bp3) to actually act to cause growth? At least when you inject free IGF-1, we know that there will be some binding (despite the short half-life) to open IGF-1 receptors. (I suggest individuals try taking their IGF-1 right after training, since damaged muscle tissue will display the greatest density of IGF-1 receptors).

 Additionally, long-R3 IGF-1 (an IGF-1 analog that’s manufactured by the Australian company GroPep), can avoid being bound to IGF-1 binding proteins and thus being inactivated. This unique ability enables long-R3 IGF-1 to prolong its serum half-life from 20 minutes to about eight hours.

More Peptide Info...

IGF Explained

IGF Explained

IGF-1 R3

IGF stands for insulin-like growth factor. It is a natural substance that is produced in the human body and is at its highest natural levels during puberty. During puberty IGF is the most responsible for the natural muscle growth that occurs during these few years. There are many different things that IGF does in the human body; I will only mention the points that would be important for physical enhancement. Among the effects the most positive are increased amino acid transport to cells, increased glucose transport, increased protein synthesis, decreased protein degradation, and increased RNA synthesis.

When IGF is active it behaves differently in different types of tissues. In muscle cells proteins and associated cell components are stimulated. Protein synthesis is increased along with amino acid absorption. As a source of energy, IGF mobilizes fat for use as energy in adipose tissue. In lean tissue,

IGF prevents insulin from transporting glucose across cell membranes. As a result the cells have to switch to burning off fat as a source of energy.

IGF also mimic's insulin in the human body. It makes muscles more sensitive to insulin's effects, so if you are a person that currently uses insulin you can lower your dosage by a decent margin to achieve the same effects, and as mentioned IGF will keep the insulin from making you fat.

Perhaps the most interesting and potent effect IGF has on the human body is its ability to cause hyperplasia, which is an actual splitting of cells. Hypertrophy is what occurs during weight training and steroid use, it is simply an increase in the size of muscle cells. See, after puberty you have a set number of muscle cells, and all you are able to do is increase the size of these muscle cells, you don't actually gain more. But, with IGF use you are able to cause this hyperplasia which actually increases the number of muscle cells present in the tissue, and through weight training and steroid usage you are able to mature these new cells, in other words make them grow and become stronger. So in a way IGF can actually change your genetic capabilities in terms of muscle tissue and cell count. IGF proliferates and differentiates the number of types of cells present. At a genetic level it has the potential to alter an individuals capacity to build superior muscle density and size.

There is a lot of talk about the similarity between IGF and growth hormone. The most often asked question is simply which is more effective. GH doesn't directly cause your muscles to grow, it works very indirectly by increasing protein synthesis capabilities, increasing the amount of insulin a person can use effectively, and increasing the amount of anabolic steroids a person can use effectively. GH also indirectly causes muscle growth by stimulating the release of IGF when it (the GH) is destroyed in the human body. So one way you could look at it as GH being a precursor to IGF. So to put it simple IGF is more effective at directly causing muscle growth and density increases. IGF is also much more cost effective.

IGF can also be effectively used by itself and gains will still be easily noticeable. With growth hormone you need to use high amounts of anabolics and often insulin to see any gains at all, this is not the case with IGF. IGF can be used by itself and is often used by bodybuilders who bridge between cycles, during this bridge is a good time to use IGF since it has no effect on natural testosterone production so it will therefore allow you to return to normal in terms of hormone levels. A stack of IGF, PGF2a, HCG, and clomid would be a good bridge stack and would allow your body to return to normal and still allow you to retain and make new gains.

IGF is a research drug, it hasn't been approved by the FDA for use as a pharmaceutical and it is currently being researched for nerve tissue repair, possible burn victims, and also as a possible aid in muscle wasting for AIDS patients. There are many different analogs of IGF available, instead of mentioning them all, I will simply mention the two most common and the most effective. Regular recombinant IGF is one of the two, it is also the more expensive and the least effective. Regular IGF only has a half-life of about 10-20 minutes in the human body and is quickly destroyed, it can be combined with certain binding proteins to extend the half-life, but it is not a very simple procedure and there is a more effective and less expensive version available. The most effective form of IGF is Long R3 IGF-1, it has been chemically altered and has had amino acid changes which cause it to avoid binding to proteins in the human body and allow it to have a much longer half life, around 20-30 hours. "Long R3 IGF-1 is an 83 amino acid analog of IGF-1 comprising the complete human IGF-1 sequence with the substition of an Arg(R) for the Glu(E) at position three, hence R3, and a 13 amino acid extension peptide at the N terminus. This analog of IGF-1 has been produced with the purpose of increasing the biological activity of the IGF peptide."

"Long R3 IGF-1 is signifacantly more potent than IGF-1. The enhanced potency is due to the decreased binding of Long R3 IGF-1 to all known IGF binding proteins. These binding proteins normally inhibit the biological actions of IGF's."

It is also not as expensive since a media grade version is available which is sufficient for bodybuilding use. There is also a receptor grade available but it is VERY expensive and the only noticeable difference between the two would only be able to be noticed in a laboratory setting. The price on the black market for Long R3 IGF-1 can be seen anywhere from $300-$500 per milligram depending on the source, be wary of black market dealers of any IGF since it is a VERY difficult item to obtain. As mentioned IGF is a research product and is only available from a few laboratories in the world and is only available to research companies and biotechnology institutions. For the rest of this article when I say IGF I am now referring to Long R3 IGF-1 for simplicity sake.

The most effective length for a cycle of IGF is 50 days on and 20-40 days off. The most controversy surrounding Long R3 IGF-1 is the effective dosage. The most used dosages range between 20mcg/day to 120+mcg/day. IGF is only available by the milligram, one mg will give you a 50 day cycle at 20mcg/day, 2mg will give you a 50 day cycle at 40mcg/day, 3mg will give you a 50 day cycle at 60mcg/day, 4mg will give you a 50 day cycle at 80mcg/day and so on. The dosage issue mainly revolves around how much money you have to spend, plenty of people use the minimum dosage of 20mcg/day and are happy with the results, and in fact several top bodybuilders use the 20mcg/day dosage and are pleased with the results. IGF is most effective when administered subcutaneously and injected once or twice daily at your current dosage. The best time for injections is either in the morning and/or immediately after weight training.

Another frequently asked question of IGF refers to the real world results, in terms of pure weight gain don't expect to gain 5 lbs. a week like you may off of anadrol or a similar steroid. The only weight you will gain from IGF use is pure lean muscle tissue, with steroids most of the weight gained is water weight. With an effective dosage you can expect to gain 1-2 lbs of new lean muscle tissue every 2-3 weeks and these effects can be increased with the use of testosterone, anabolic steroids, and insulin use. Increased vascularity is also very common, people report seeing veins appear where they never have before. And yet another effect reported is the ability to stay lean while bulking with heavy dosages of steroids and TONS of food while on an IGF cycle, this is perhaps the most pleasing effect. Increased pumps are also noticeable almost immediately, the pumps can almost become painful, pumps are even noticeable when doing cardio.

Overall, IGF is a very exciting drug due to its ability to alter ones genetic capabilities. If you can find a trustworthy source and you use it correctly it can be a VERY useful tool in your bodybuilding drug arsenal

IGF1, also known as somatomedin C, is polypeptide hormone about the same size as insulin. It is produced predominantly in the liver in response to growth hormone (GH) release from the pituitary gland. Many of the growth promoting effects of GH are due to its ability to release IGF1 from the liver. The conversion ratio of GH to IGF1 varies greatly in different individuals but most external sources of GH convert around 4-6mcg of IGF per one I.U. of GH. IGF-1 acts on several different tissues to enhance growth. IGF1 belongs in the 'superfamily' of substances known as 'growth factors,' along with epidermal (skin), transforming; platelet derived fibroblast, nerve, and ciliary neurotrophic growth factors. None of the other factors have any bearing on exoskeletal tissue incidentally however These agents all have in common the ability to stimulate cell division, known as mitogenesis, and cell differentiation. Meaning That In the case of IGF1 which does act on muscle tissue it will initiate the growth of new muscle fibers, and subsequently new receptors for testosterone. Users have unanimously concluded that it enhances cycles of steroids significantly. They also seem to be adamant about its ability to reduce fat and improve vascularity a great deal.

 

The IGF-1 Hype

There is a considerable amount of hype surrounding IGF1. Every one is blaming the distended bellies of modern Bodybuilders on it. Also the freaky proportions that old bodybuilders that have been around for years are starting to attain. Anti-aging proponents are touting it as the miracle cure for every thing from Parkinson's disease to Alzheimer's. And the medical community has published numerous articles on it for its ability to cause cancer, diabetes and gigantism. While at the same time performing documented experiments on thousands of patients of muscle wasting diseases. And reporting significant turnabouts in there conditions. So what is a guy to think about IGF1 as far as athletic enhancement is concerned? Well first of all you need to know that most experiments conducted with IGF1 do not list the type of IGF used. I have written Dr. Robert Saline of the Swedish rejuvenation institute on several occasions and we have had in-depth discussions on the subject of IGF1 for physical appearance enhancement. He feels it would be unethical to prescribe IGF1 to a bodybuilder to increase muscle mass simply due to the fact that IGF1 has valid applications in the medical community, (Like I could give a rats ass about "ethical"). He can not argue that it is extremely effective as a promoter of muscle growth far beyond what androgens (steroids) alone can offer. Well fortunately in America IGF1 is not a drug (yet) and the FDA has no control over it as of now. This will change in the very near future however, Im absolutely sure of it.

How to use IGF-1

Assuming that you have acquired legitimate IGF1 (R3) long chain, That's IGF1 with the binding protein added. You should take dosages ranging from 60mcg up to 120mcg per day in divided doses. One injection in the morning and again at bed time. Never exceed 120mcg in one day. IGF1 can cause serious gastrointestinal problems such as tumors intestinal swelling diarrhea and vomiting. Most IGF1 comes in a concentration of 1000mcg per ML or CC so it makes it easy to measure in an insulin syringe. 10 IU on the syringe is 100mcg. Do the math.

IGF + Insulin

If you plan on doing IGF1 with Insulin, listen closely IGF1 is not that expensive, sure you can get away with using less by including insulin in the stack, but IGF1 and Insulin together have a pro-insulin effect on your blood sugar balance. It can enhance the chances of a hypoglycemic episode ten fold. I would recommend against it for any one not ABSOLUTLY comfortable with insulin or IGF1.

Here is how insulin and IGF1 work together. Igfbp3 is the binding protein, which allows IGF1 to remain active in the system for a long enough period of time to really work its magic. IGF1 by nature has a half-life of less than 10 minutes by its self. The molecule was so small it would escape the blood stream very rapidly. This was the reason IGF1 was so "underground". It took very frequent injections at high dosages to achieve even minimal results. Aside from this reconstituting the compound required a degree in biochemistry. This short acting version was the only IGF1 known until recently IGF1 would have been administered in 100 mcg dosages 4-6 times a day. That is a hell of a lot of IGF1. That explains a lot of the distended bellies. Now with R3 long chain IGF1 and the Binding protein IGFBP3 IGF1 will last up to 6 hours in the system. By binding IGF to the IGFBP3 you make the molecule larger and it gets trapped in the blood stream until the protein is broken down and the IGF molecule escapes. You can further its life by combining Insulin with it, although I here its very risky. Insulin prevents the breakdown of IGFBP3 and leaves the IGF1 molecule roaming free in the blood stream for longer periods of time up to 12 hours as insulin levels return to normal IGFBP3 will begin to break down and the IGF1 will escape from its bound protein IGFBP3 again having a half life of less than 10 minutes.

Insulin should be taken at the normal dosage it is usually administered at minus 10% about 45 minutes prior to the IGF1 infusion. Again let me remind you this can be deadly if you don't know what you are doing. And of course do not use Insulin for the nighttime injection of IGF1 by taking it in the morning you prolong the IGF1's half life to 12 hours and then take a 6 hour injection, you should be fine. Hell if you want to eat a big bowl of rice and drink another 100g of simple carbs 45 minutes before the bed time IGF1 infusion you could spike insulin for at least a few hours of extended IGF1 activity. If your not going to be using insulin in the stack then go ahead and do the same in the morning.

What users report

Users of IGF1 have reported various results but all along the same lines, It does not appear to be dramatically less effective in any one individual (at least not to the best of my knowledge). I have a good friend who had to stop taking IGF1 due to stomach illness that was completely unrelated But he to experienced good gains from it for the 2 weeks he was on it, his dosage was 120mcg per day. One hour after the first injection he went to the gym and immediately told me about the uncontrollable pump he got from just one set.

That would indicate to me that he was experiencing some form of cell volumization. The general consensus on IGF1 seems to be that its benefits are as fallow:

Increased Pump Pumps are reported to be so severe that workouts are often cut short due to lack of ability to the muscle through the full range of motion...ouch

Gains retention is increased if IGF is used in a cycle I am not sure why, but IGF1 seems to make gains on a cycle stick with virtually no post cycle loss. Every bodybuilder I've spoken with seems to think this for some reason. Most of them use drugs like Anadrol or Dianabol with it because of the amount of size attained with these drugs. The usual draw back to these drugs is that in most users there is a post cycle "crash" that occurs, so the reasoning is to toss IGF1 into the stack and grow larger faster with out the post cycle crash blues.

Reverses testicular atrophy

Testicles if shrunken will return to "full swing" so to speak even in the middle of a cycle. If not shrunken they will not shrink during the cycle. This may explain partially why gains are kept after the cycle.

Fatigue

Users report feeling drained and tired all day. This seems to be one of the negative side effects to IGF1, it will make you sleep longer and you will require more sleep at night to feel rested for the morning. This is common with high doses of HGH and exhibited in children, whose IGF1 levels are extraordinarily high. A child needs 4 hours more sleep than an adult on average does. This may be directly or indirectly related to IGF1 levels.

Stiffness

An almost arthritic feeling is commonly associated with high levels of HGH, well IGF1 has the exact same property. IGF1 will cause your hands, fingers and knuckles to ache this is one way you can be sure you got real IGF1.

IGF-1's Side effects

Every thing has a down side. To bake a cake ya gotta brake an egg. IGF1 is no exception. The drug used in larger quantity around the 100mcg+ range will cause headaches, occasional nausea and can contribute to low blood sugar or hypoglycemia in some users. Although I have never heard of this first hand I'm sure its true.

IGF1 will attach its self to the lining of the intestine and cause atrophy of the gut. Every thing IGF1 touches will grow and you have a lot of receptors on the lining of the large intestine and inner wall of the abdominal well. This is what causes the GH gut look. You can easily avoid this by limiting your dosages and cycle lengths. IGF1 cycles should be kept to 4-6 weeks with 4-6 weeks off in-between. IGF-1 is considerably more powerful than HGH and you need to think of it along those lines as far as dosing goes. We all know what to much HGH can do over prolonged periods of usage. The Neanderthal look is definitely not going to win any shows this year. I would recommend 80 mcg a day for 4 weeks at a time you should get good results from that for a while. I don't know if you will need to up the dosage at any point, but I would think in the case of IGF1 it wouldn't matter. If 80mcg doesn't do it for ya, then bump it up to 100 You should definitely feel it at this point If not suspect the IGF1 as being fake. Beyond 120 mcg per day your asking for trouble, This compound demands as much respect as its sister amino Insulin.

Clinical Facts about IGF-1

IGF-1 is a polypeptide of 70 amino acids (7650 daltons), and is one of a number of related insulin-like growth factors present in the circulation. The molecule shows approximately 50% sequence homology with proinsulin and has a number of biological activities similar to insulin. IGF-1 is a mediator of longitudinal growth in humans or how tall you are capable of becoming. Serum IGF-1 concentrations are altered by age, nutritional status, body composition, and growth hormone secretion. A single basal IGF-1 level is useful in the assessment of short stature in children and in nutritional support studies of acutely ill patients. For the diagnosis of acromegaly, a single IGF-1 concentration is more reliable than a random hGH measurement (Oppizi, et al., 1986). IGF-1 can be used for the assessment of disease activity in acromegaly (Barkan, et al., 198.

Almost all (>95%) of serum IGF-1 circulates bound to specific IGF binding proteins (IGFBPs), of which six classes (IGFBPs 1-6) have been identified (Rudd, 1991). BP3 is thought to be the major binding protein.

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Basic peptide guide

Basic peptide guide

Peptide Information

Insulin-like Growth Factor LR3 (IGF-1)

IGF-1 LR3 Dosage(Long R3 IGF-1):
Light: 25 mcg
Common: 50 mcg
Large: 100 mcg
IGF-1 is administered for one month, give or take a week.
Insulin-like Growth Factor (Long-R3 IGF-1), an 83 amino acid analog of IGF-1 is a highly anabolic hormone released primarily in the liver with the stimulus of growth hormone (HGH).

IGF-1 is the most potent growth factor found in the body and causes muscle cell hyperplasia.
Freeze dried (lyophilized) IGF-1 (in powder state) should be stored in the freezer (-18 degrees Celsius).
Example Long r3 IGF-1 kit contains:
1000mcg of lyophilized (freeze dried) Long R3 IGF-1
2 mls of 0.6% Acetic Acid (AA)
30ml Sodium Chloride (NaCL) as buffer
Dilute the IGF-1 peptide with 2mls of Acetic Acid (.6%). Assuming (*DO NOT ASSUME*) Acetic Acid (AA) will yield the correct pH balance of your research peptide.
Note: This creates a concentration of 500mcg/ml. So each 1/10 of a ML is 50mcg’s.

Draw the desired amount of IGF in to a syringe. Desired amount should be the approx. dosage wanted.
Example- 2mls AA used to reconstitute IGF-1 1mg vial means 5 units on a U100 insulin syringe would equal 25mcg IGF-1 LR3
Pre-load your syringes at 5iu (25mcg IGF-1). Divide your IGF-1 into 40 syringes for storage in the freezer.
Thaw prior to administration. Draw from your NaCL after thawed to buffer (.5ml is enough).

Unknown whether injecting IGF-1 to increase muscle growth is efficacious. Many believe in the value of this powerful growth factor.
It is possible to go into hypoglycemia fro IGF-1 supplementation. Effect is dose dependent.

(information in this thread gathered via the internet)


Melanotan peptides, PT-141, CJC-1295, GHRP-6, IGF-1 LR3, GH fragments, come in the form of freeze dried powder using Bacteriostatic Water (BW) for reconstruction and preservative. Research peptides are supplied in multiple-dose vials from which repeated withdrawals may be prepared for subcutaneous injection (administrated intramuscularly in rare instances).

 Melanotan 1 - Melanotan is a synthetic (potent) version of melanocyte stimulating hormone (a-MSH). Afamelanotide treatment induces skin pigmentation through melanogenisis, the production of melanin. Melanin prevents cellular damage in the skin by absorbing, reflecting and refracting light. Melanotan reduces sun damage to UV exposed skin in those with sun allergies.

Melanotan 2 - Melanotan II is a cosmetic product that stimulates a natural increase in melanin production. Melanin is the main determinant of skin color in humans, a brown pigment which causes skin to become darker in appearance, instead of red, when exposed to UVR. Users of Melanotan II develop a gradual, natural tan with minimal sun exposure. Melanotropins useful for all skin types, particularly fair-skinned (Fitzpatrick skin type 1 & skin type 2).

Bremelanotide (PT-141) - Unexpected sexual arousal experienced by Melanotan 2 tanning trial subjects led to development of Bremelanotide PT-141. PT-141 does not act upon the vascular system, but the nervous system (cacading direct from the brain). Bremelanotide results are the world's only synthetic aphrodisiac developed after Melanotan 2 (MT-II).
 
GH Releasing Peptide-6 - GHRP 6 is a synthetic hexapeptide (six amino acid peptide) that releases growth hormone release. High levels of growth hormone have been associated with fat loss, muscle gain, general well being and anti-aging effects. Ghrelin effect from GHRP-6 can cause hunger. Ipamorelin, Hexarelin and GHRP-2 also bestselling GHRP research peptides.

GHRH (CJC-1295) - CJC-1295 (GHRH) continuously elevates Human Growth Hormone (HGH) and IGF-1. In the healthy body, large amounts of growth hormone are stored within the pituitary. CJC-1295 DAC has been proven to stimulate slow wave sleep helping restore the body. Research Sermorelin, CJC-1293, CJC1295, Mod GRF 1-29 (best) research peptide.

 Insulin-like Growth Factor LR3 - Insulin-like Growth Factor (Long-R3-IGF-1), analog of IGF-1 is a highly anabolic peptide released primarily in the liver with the stimulus of growth hormone. IGF-1 LR3 and IGF-1 DES popular glucose disposal agent analog peptides sold online. Dosage for IGF1 LR3 unspecific, whereas quick acting IGF DES pre-exercise administration recommended.

 191AA Growth Hormone (HGH) - Human Growth Hormone (rHGH, HGH, or GH) is a protein molecule consisting of 191 amino acids. Genetically engineered recombinant growth hormone is completely identical to the growth hormone made by the human pituitary gland. Growth hormone helps body composition and is rapidly converted to its powerful growth promoting metabolite, Insulin like Growth Factor (IGF-1).

 PEPTIDE INFO

Peptide Information

Peptides come in the form of lyophilized (freeze dried) powder. The amount of powder/product is stated in International Units (IU's) or in Milligrams (MG).
Melanotan peptides (Melanotan 1 & Melanotan 2), PT-141 Bremelanotide, GH Fragment, Ipamorelin, CJC-1295 & GHRPs (GRHP-2 & GHRP-6), HGH, HCG, et cetera use Bacteriostatic Water (BW). Bacteriostatic Water for injection, USP is a sterile, nonpyrogenic preparation of water for injection containing 0.9% of benzyl alcohol added as a bacteriostatic preservative. It is supplied in a multiple-dose container from which repeated withdrawals may be made to dilute or dissolve drugs for injection. The pH is 5.7 (4.5 to 7.0)

For IGF use an acetic acid solution (.6%) which is 7 parts distilled water and 1 part vinegar to reconstitute. You must filter the distilled water and white wine vinegar through a sterile 20 micron syringe filter before use. Sodium Chloride (NaCl) is used to buffer the injection.

1.) Take an alcohol swab to the stopper of both your peptide vial and the vial of the diluent.

2.) Draw your preferred diluent (BW) with a 1cc syringe. Choose an amount that will make measuring the final product simple.

1ml(cc) per 10 mg vial of Melanotan would mean each 10 tick marks on a U100 slin syringe would equal 1mg of Melanotan
1ml(cc) per 10 IU vial of HGH would mean each 10 tick marks on a U100 slin syringe would equal 1 IU of HGH

3.) Take the syringe with the diluent and push it into the vial of lyophilized powder letting the diluent dissolve the peptide. Many (not all) peptides are sealed with vacuum pressure, be careful.

4.) After diluent has been added to the vial, gentling swirl the vial until the lyophilized powder has dissolved and you are left with a clear liquid. The peptide is now reconstructed, ready for measurement and usage.
5.) Store your now reconstituted research peptides in the refrigerator.
Peptide Measurement
After successfully reconstituting your peptide, measure the desired amount out for injection. Use a U100 insulin syringe to draw out and inject your product.

Since you know the amount of IU's/MG's in your vial, we divide this out as follows:
You will need to know the following to be successful: 1ml = 1cc = 100 IU's

We take our dose from the label of the dry lyophilized powder and we divide that into the amount of diluent used.

Example- We used 1cc(ml) of water. We have a 10 IU vial of HGH.
From our formula above we know that 1cc = 100 IU's, so we have 100 IU's of water.
We now divide the 100 IU's (the amount of our water) by 10 IU's (the amount of our HGH)
100 IU / 10 IU = 10

This 10 will perfectly correspond with the markings on a U100 insulin syringe. In our example every 10 mark on our syringe will equal 1 IU of HGH. Want to draw out 2 IU's of GH? ....draw out to the 20 mark on the syringe (1/5th of the syringe).
Say you have a 1mg vial and you add 1ML you get
1000mcg/1mL: 10 mcg per IU
1000mcg/2mL: 5 mcg per IU
Say you have a 10mg vial and you add 1ML you get
10mg/1mL: 1 mg per 10 IU
10mg/2mL: .5 mg per 10 IU
Say you have a 20mg vial and you add 1ML you get
20mg/1mL: 2 mg per 10 IU
20mg/2mL: 1 mg per 10 IU
Say you have a 10iu vial and you add 1ML you get
10iu/1mL: 1 iu per 10 IU (on the syringe - 1/10th the product)
10iu/2mL: 1 iu per 20 IU (on the syringe - still 1/10th the product)
Say you have a 5000iu vial and you add 1ML you get
5000iu/1mL: 500iu per 10 IU
5000iu/2mL: 250iu per 10 IU

 Melanotan  1

Afamelanotide (M-I) Dose:
Light: .5mg
Common: 1mg
Large: 2mg
Melanotan 1 synthetic melanocyte stimulating hormone (a-MSH) induces skin pigmentation through melanogenisis, melanin production. Melanin prevents cellular damage in the skin by absorbing, reflecting and refracting light. Melanotan reduces sun damage to UV exposed skin transforming cells to produce dark protective melanin.

Melanotan Results: Melanotan favors production of eumelanin (black/brown) over pheomelanin pigment. Injecting this linear amino acid Melanotan 1 peptide offers super-physiological levels of tanning ability.

 Melanotan 2

Melanotan 2 Dose:
Begin: 100mcg
Light: 250mcg
Common: 500mcg
Stout: 1mg
Large: 1.5mg
Max: 2mg
What is Melanotan 2
Melanotan II analog of alpha-melanocyte stimulating hormone (a-MSH). Melanotan peptides are safe and efficacious with growing long term data. Melanotan 2 is a freeze dried peptide sealed in a sterile multi-use vial. Generic 10mg Melanotan II:

Melanotan injections act on melanocytes to stimulate melanin production. Melanin is the body's pigment responsible for a photoprotective safe tan.

Bremelanotide

Bremelanotide PT 141 Dose:
Light: .5mg
Common: 1mg
Large: 2mg
Bremelanotide PT-141 was developed from Melanotan 2 (MT-II). PT-141 is a metabolite of melanocyte stimulating hormone that targets desire.

Treatment: PT-141 is the only synthetic aphrodisiac. The aphrodisiac effects of Bremelanotide are in a class of its own. Studies have shown Bremelanotide to be effective in treating sexual dysfunction in both men (erectile dysfunction or impotence) and women (sexual arousal disorder). Nine out of ten volunteers experienced sexual arousal in clinical trials. Unlike Viagra and other related medications (PDE5s - blood pushers), PT-141 acts upon the nervous system. Viagra, Cials and Levitra are not considered aphrodisiacs as they do not have any direct effect on the libido. However, treatment with PDE5 inhibitors and PT-141 have known synergy.

Men's Journal Magazine: ...it took hold. I felt a great surge of affection (greater than any regular level of arousal). My body tingled and I developed an erection that wouldn’t quit. For two hours the drug wouldn’t let me out of its grasp — nor my wife out of mine. -Frederick Kaufman

Females: Women who took part in trials said that they felt a "tingling and a throbbing" along with "a strong desire to have sex." An initial flush occurs post injection, followed by nausea which is dose dependent. For most, effects generally do not take place until a couple hours post injection, peaking around the four hour mark. Men said PT-141 made them feel "younger and more energetic" as well as sexually interested and aroused. "You're ready to take your pants off and go," said user "a product that makes you not only able to but eager to."
Bremelanotide PT-141 Peptide: Bremelanotide comes in 10mg vials. As a lifestyle peptide, the 10mg product is a lot to consume and may offer up to 20 doses when dosing conservatively. Research PT-141 reconstituted with bacteriostatic water remains potent in the fridge for months.
Window of Opportunity: Bremelanotide, injected (subcutaneously), has a unique window of opportunity lasting six to 72 hours. In lab trials female rats exposed to PT-141 began "flirting" with male rats for sex. Postures and movements left no doubt in the male rats minds that they were in the mood. The human PT-141 date is one where the dosage precedes the activity by at least a couple hours. When the stars align, hours after the injection, ...this is your window of opportunity, enjoy.
Mixing: Bacteriostatic water is used for reconstitution.
Example- 1ml(cc) bacteriostatic water per 10mg PT-141 vial equates to a 1mg dose approximately each 10 units on a U100 insulin syringe.
Example- 1ml(cc) bacteriostatic water per 2mg PT-141 vial equates to a 1mg dose approximately every 50 units on a U100 insulin syringe.
Recommended strategy for mixing and dosing would be to reconstitute with the volume that yields a .1ml injection.
Example- 1ml(cc) bacteriostatic water per 10mg PT-141 vial equates to a 1mg dose approximately each 10 units on a U100 insulin syringe.
Example- .2ml(cc) bacteriostatic water per 2mg PT-141 vial equates to a 1mg dose approximately every 10 units on a U100 insulin syringe.
Dosing: Read as much as possible to gain clarity and align expectations. Gradually dosing increases likelihood for success without sides. A test dose of .25-.5mg on the first attempt is recommended. 1mg, give or take a quarter, is the efficacious dose which yields the most positive reports by users.

Ghrp-6

GHRP-6 Hexapeptide Dosage:
Light: 50mcg
Common: 100mcg
Large: 150mcg
Growth Hormone Releasing Peptide (GHRP-6) is a peptide in the growth factor family. It has strong effect on the release of Human Growth Hormone (HGH) in a specific and dose-related manner. GHRP can be effectively used in the treatment of growth hormone (GH) deficiency. Growth hormone releasing hexapeptide works by signaling the pituitary gland to begin growth hormone secretion.

Increased GH and IGF-1 levels are desirable for those looking to improve physique. Human growth hormone has been known to enhance immune response and stimulate the immune system, particularly older subjects. Dosed at night for anti-aging purposes and multiple times throughout the day for anabolism. GHRP is often used in conjunction with GHRH CJC-1295 (mod GRF 1-29) to amplify growth hormone pulse. Avoid fats and carbohydrate a half hour on each side of dosing GHRP-6.

Bodybuilders and athletes dose GHRP in an effort to build more muscle and burn fat. Some GHRP-6 users include it in their post cycle therapy (PCT). Cycling GHRP-6 in the off weeks from IGF/GH cycles is also becoming prevalent. Researchers wish to kick-start their body into producing their own natural GH & IGF, while gaining as if they remained on the GH/IGF peptides.

GHRP-6's main use is to promote food intake by stimulating hunger and aid in energy metabolism. The major side effect being a significant increase in appetite due to a stimulating the release of Ghrelin (about 20 minutes post injection), a hormone released naturally in the lining of the stomach and increases hunger and gastric emptying. This is why GHRP-6 can be used in the treatment of cachexia (wasting), eating disorders and obesity.

Benefits of increased HGH levels through GHRP-6 stimulation include: an increase in strength, muscle mass and body fat loss, rejuvenation and strengthening of joints, connective tissue and bone mass. Enhanced HGH secretion also leads to the liver secreting more IGF-1, which is thought to be the primary anabolic mechanism of action for Growth Hormone.
Bodybuilding Peptide GHRP-6:

Mixing: Bacteriostatic water is used for reconstitution. When diluted, peptide lasts a very long time when left alone in the refrigerator (months)

Example- 2.5ml(cc) bacteriostatic water per 5mg GHRP vial equates to a 100mcg dose approximately each 2-3 marks on a U100 insulin syringe.
Example- 5ml(cc) bacteriostatic water per 5mg GHRP vial equates to a 100mcg dose approximately every 5 marks on a U100 insulin syringe.

Dosing: The saturation dose of GHRP-6 has been determined to be around 100mcg. More is not better in regards to this secretagogue

5mg GHRP = 5,000mcg

5,000mcg/100mcg = 50 100mcg GHRP doses per 5mg

Growth Hormone Releasing Hormone (CJC-1295)

Modified GRF 1-29 (GHRH) CJC1295 Dosage:
Light: 25mcg
Common: 50mcg
Large: 100mcg
Growth Hormone Releasing Hormone (GHRH) CJC-1295 is a synthetic secretagogue which stimulates HGH release. In the human body, large amounts of growth hormone are stored in the pituitary. GHRH affects the number of secreting cells and the amount they are able to secrete. GHRH Analog research peptides sold online are a fantastic addition to GHRP (GHRP-6 & GHRP-2).

Dosing GHRH once at night for anti-aging purposes and multiple injections (1-3x) for anabolism. CJC-1295 has the ability to make the body produce its own GH as compared to using synthetic HGH. GHRP-6 use in conjunction with CJC-1295 is synergistic, amplifying the GH pulse considerably. Synthetic GH side effects can be experienced with pure growth factor peptides. Researchers also report positive effects much like that of HGH. Particularly energy, quality sleep, fat loss/metabolism, improved skin quality, pumps in the gym and numb/tingles at extremities.

CJC-1295 is typically found in 2mg vials. Reconstitute with BW. Inject subcutaneously. Generally best to invest in shorter acting modified GRF 1-29 as CJC-1295 manufacturing is difficult and fragile. GHRH (mod GRF 1-29) dose has 30 minute half life, superior to that of Sermorelin. Post injection flush is common. Ipamorelin is increasingly popular research peptide alternative. Genuine CJC-1295 DAC bloats male researchers, best to avoid real CJC-1295.

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