Human growth hormone
Human growth hormone (hGH) is a single-chain polypeptide that participates in a
wide range of biological functions such as metabolism of proteins, carbohydrates
and lipids as well as in growth, development and immunity. Growth hormone
deficiency in human occurs both in children and adults. The routine treatment
for this condition is administration of recombinant human growth hormone (rhGH)
made by prokaryotes. Since nonglycosylated human growth hormone is a
biologically active protein, prokaryotic expression systems are preferred for
its production.Different strains of E.coli were transformed by plasmid
containing human growth hormone gene and cultured in different conditions. After
induction by IPTG, recombinant human growth hormone production was assessed
using ELISA, dot blotting and western blotting techniques.High levels of rhGH
were produced using E.coli prokaryotic protein production system.This simple and
cost effective production process could be recruited for large scale production
of rhGH.
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