Understanding Peptide Reconstitution Guide

Understanding Peptide Reconstitution

It seems to be increasingly common that users are introducing themselves to the idea of pinning through the use of peptides (although, arguably, hcg usage is also increasing in usage for those running non-injectable cycles). While I personally find these to be a great way to get yourself comfortable with the idea of injecting, it seems many become confused nearly immediately with how to at the first step, reconstitution. So I decided to just create a quick reference for those of you out there, and show you how the simple math required is not intimidating in the least. This guide will be a bit long, as I am hoping to avoid the common questions, but I think it will be a very good guide for some, and maybe too "simple" for others.


 A short disclaimer:
 This guide is purely hypothetical, and I do not encourage the use of any PED without the close guidance of a physician. This guide takes no consideration to the laws of your country. I do not condone any illegal activity. It is merely designed to be an educational resource.




 Alright, so this guide will follow under the assumption that you have aquired your peptide of choice (or hCG), and are ready to reconstitute it.


 You will need the following items:
•Your Peptide Vial
•Bacteriostatic Water
•Alcohol Wipes (available at your local pharmacy, usually near diabetic supplies)
•Insulin Syringe (more on this in a moment)

 A quick discussion on syringes:

 Syringe availability will vary largely depending on the laws of your state/country. In many states, such as mine, low quantity purchases of insulin syringes are available at the pharmacy without the need for a prescription. Laws such as these are in place as a counter measure to help avoid the increase of HIV transmission through unsafe needle usage, by allowing addicts to purchase clean syringes.


 Size will depend on your source. An insulin syringe will typically range from 29g to 31g. For those unaware, the smaller the gauge (the "g"), the larger the number. So a 31g will be smaller needle width than a 29g. Generally, smaller gauge needles can provide less soreness through injection, but they are also much more fragile. Having used a 31g, I do prefer them for the ease of injection (minimal "pinch" feel), but they do become increasingly fragile, so proper care is required.


 Now that you have your supplies gathered, it is time to reconstitute your peptides. Please make sure that you do this in a clean environment to reduce the risk of contamination.


 For this example, I will be using a typical 5mg product, as is typical of the GHRPs provided by many companies. Although your particular product may not contain 5mg, the same idea will hold through to other products, just with a different amount of actives. I will also be under the assumption that you will want 100mcg doses (although again, this will vary by product/your required dosing).

Before you begin, you must do the math to understand how much bacteriostatic water you will need for your reconstitution.


 If you are looking for the said 100mcg doses, from a 5mg vial, then you will have 50 doses from this vial. 100mcg x 50 = 5,000mcg, or 5mg. You can apply this to any amount of product. For example, Ipamorelin is usually available in 2mg vials. In that case, you would have 2,000mcg of Ipamorelin, which divided by 100mcg would give you 20 doses. The amount of dosages is important here, as it will dictate how to utilize your reconstituted product.


 So back to the original 5mg product, we know we are going to have 50 doses. The idea here then, is to add enough bac water to the peptide in order to allow you to dose those individual dosage. Personally, I like to use as little Bac water as needed, but also attempt to keep it easy enough to dose. The key point here is to know that no matter how much bac water you add to your vial, 1/50 of that will always be 100mcg.


 With that said, if you add 1ml of bac water to the vial, your 100mcg dosage will be 2iu on the syringe.
 (1ml = 1cc on your syringe = 100iu, so 100iu/50 doses = 2iu).
 If you add 2ml of bac water to the vial, your 100mcg dosage will be 4iu (200iu/50 doses = 4iu).
 If you add 3ml of bac water to the vial, your 100mcg dosage will be 6iu (300iu/50 doses =6iu).
 It is up to you how much bac water you will like to use, and utilizing the option of keeping this guide non-opinionated, I won't give a recommendation on this, however, it will be dictated by how large your vial is and how accurate your pins are (a 50iu *sold as 1/2 cc* syring will make it easier to measure smaller amounts, where a 1cc *100iu* syringe will make it more difficult to measure smaller amounts).


 Once you have an amount chosen, we can move on the the actual reconstitution. In this example, I will reconstitute with 2ml of bac water.


 1. Snap of off the plastic cover of your peptide vial and your bac water.

2. Take an alcohol wipe, and wipe down the rubber top of both the peptide vial and your bac water, and then discard the wipe and allow vials to dry for a second or two.


 3. Take a syringe and remove the safety cap (most will have one cap over the needle, and one over the plunger). Set these caps aside, while taking precaution not to expose your needle to any other object (it is sterile!). Pull the plunger on your syringe to the maximum reading (will usually be either 1/2cc or 1cc) to allow the syringe to fill with air. (It is also common practice to draw this air from the peptide vial. You can do this by inserting the needle into the peptide vial, keeping it right side up, and drawing the air that way. Then pull the syringe from the bottle, and on to step 4).

4. Insert the needle into the rubber portion of your bacteriostatic water. With the syringe inserted, turn the vial of bacteriostatic water upside down, and push the plunger into the syringe (this will cause the air to enter the vial). Then, slowly, draw back on the syringe back to that maximum reading, as it will allow the syringe to fill with bac water.
 Quick Note: The reason you fill your syringe with air, and then insert the air into the vial, is to keep the pressure within the vial, which will allow you to draw with much greater ease.

5. You may now turn the vial of back water back to right side up, and remove your syringe, again taking caution to avoid the needle from coming into contact with anything else.

6. We are now ready to insert the bac water into the peptide vial. I recommend reconstituting slowly, and avoiding adding the bac water directly onto the peptide, and rather down the sides of the vial. Take your peptide vial, and insert the syringe through the rubber stopper on an angle, enough so that once the needle penetrates the rubber, it is angled towards the side of the vial.


 7. Slowly push down on the plunger allowing the bac water to enter down the sides of the vial. Take your time doing this, as there is no rush.

8. Once all of the bac water is into the vial, remove the syringe and gently (GENTLY!) swirl the vial allowing the peptide to dissolve into the bac water.

9. Depending on the target amount of bac water to be added, and the size of your pin, you must now repeat steps 3-8 until you have added the correct amount of bacteriostatic water. If you are aiming for 2ml of bacteriostatic water, and are using a 1/2cc syringe, this process must be done a total of 4 times, and if using a 1cc syringe, this process must be done a total of twice.

10. Once the proper amount is added, place the caps back on both ends of your syringe and properly discard of it. Do not reuse this syringe for injection.
 Depending on your product, refridgeration might be necessary. If so, store reconstituted product in fridge.

That should be it. Happy research. If you have any questions, feel free to ask, but only do so after having completely read through this guide.
 If you guys like this guide, I might do some later on some common peptides, when I can get to it.
 -h2s

IGF-1 Reverses Aging and Heals damaged brain cells

Growth Hormone Production and IGF-1The production of growth hormone is at its peak during the adolescence period. Most of the HGH is secreted into the bloodstream in brief-bursts manner. It also occurs during the early hours of deep sleep or REM. Once HGH is in the bloodstream, it stays there for a short period, perhaps in a few minutes or longer enough in stimulating its uptake into the liver, wherein conversion to growth factors happen. The Insulin Growth Factors (IGF-1) are the most important of all these growth factors, also called the Somatomedin C.

Furthermore, the growth hormone acts either indirectly or directly through the intermediary insulin growth factors to each organ system in the body. Hence, almost nothing is able to escape from its magic touch. Growth hormone is also capable of increasing the size of several organs and tissues, including the brain. Based on recent studies, involving animals as test subjects, HG has manifested the regeneration of damaged brain tissue.

IGF-1Rather than the growth hormone itself, it is the IGF-1 that varies widely throughout the day and is used in measuring how much GH has been secreted by the body. Thus, the IGF-1 is responsible for the actions and benefits associated with HGH and is 10 times more potent than HGH. To date, HG is under investigation as a separate medication for its many indications similar to HGH. Phil Micans from the London-based International Aging Systems, believes that the IGF-1 will become the top choice among the hormones in just a few years.

HGH and IGF-1 for Anti-aging Doctor Vincent Giampapa, the Clinical Research director of the Longevity Institute International, based in Montclair, New Jersey, said that the blueprint of aging is under the DNA’s hood of telomere, and considered as the “clock” at the end of each chromosome, which is shortened with every cell division.

Hence, reversing the aging at the cellular level is possible through a substance, which will restore the length of telomere. However, that substance is still unavailable. Giampapa strongly believes that the substance will be available in not more than a decade though. Until then, the growth hormone, along with its IGF-1 attendant hormone is able to perform the next best thing, assisting in keeping the cell as healthy as possible.

Overall, the ability and function of the cell largely depend on the DNA, the genetic material in the nucleus that codes for all the enzymes, proteins, and hormones that make it run. Thus, DNA is similar to an army under a consistent attack from ultraviolet light, body heat, oxygen free-radicals, and many other damaging factors. While the DNA has the ability in repairing itself, it still falls short with age, which is the same aging process that largely affects the cell. Simultaneously, damage accumulates in the cell’s mitochondria, the energy center, which has its separate DNA. Among the things to limit the DNA damage is the intake of antioxidant supplements like vitamins E and C in bolstering the body’s defenses.
According to Giampapa and a hormone replacement therapy specialist Thierry Hertoghe, the latest research in Europe indicates that human growth hormone and the IGF-1 could go farther than antioxidants, going beyond what antioxidants can do. All the more, HGH and IGF-1 can transport raw materials to the cells for repair and renovation.
IGF-1 can instigate the delivery of RNA, DNA and nucleic acids directly into the cell nucleus. Meanwhile nucleic acids are helpful in repairing the damage in the DNA, while stimulating cell division. Growth hormone, on the other hand, launches the transport of nucleic acids and amino acids into the cell’s cytoplasm, just outside the nucleus. In this process, the HGH and IGF-1 are not only able to minimize the DNA and cell stellar damage, but also treating the DNA and the cell. In all, both hormones can treat the blueprints of the aging process.

Primarily, the IGF-1 is the GH chain’s other end, the downstream player, which actually exerts the effects associated with the HGH. It is able to cause a great deal of excitement in two groups, including the researchers and the group of bodybuilders, who are both using the substance for individual purposes.
Meanwhile, the IGF-1 is said to be more potent than the HGH, as it latter exerts its effects through the former substance. Without a surprise, IGF-1 injections can do what HGH does, plus other effects in accordance with its proponents. IGF-1 can increase the lean body mass, while reducing fats, building bone, nerves, and muscle all at the same time. Through a direct intake, the pituitary gland is avoided, which could be burnt out throughout the aging process.

Anti-Aging Action – When it comes to the anti-aging efforts, the iGF-1 is said to be more potent than the HGH. Doctor Keith Kelly conducted a research work, indicating that growth hormone was able to reverse the shrinking of the thymus. According to Kelly, only the IGF-1 worked, and not the GH that worked in his experiment on cells in culture. However, both GH and the IGF-1 worked on living animals, saying that both substances are significantly elevated in the older test subjects as they were treated with growth hormone. He predicted that IGF-1 would be the main player though.

Disease and Brain Aging Prevention – Among the other spectacular uses of GH and IGF-1 is their ability to prevent the effects of brain aging, not to mention their capacity to treat such condition. Based on an experiment, the substances manifested momentous implications for stroke, brain injury, neurodegenerative disease, and aging. A team of scientist based in New Zealand said that IGF-1 can halt the death of cells in the brain.
Peter Gluckman, Barabara Johnston, along with their colleagues at the University of Auckland discovered that IGF-1 injections administered in two hours, following brain injury in baby lambs were able to rescue the damaged neurons, salvaging cells that would have died during apoptosis, the programmed cell death. Apoptosis is the brain cell loss that occurs for up to three days upon the initial injury.
In all, the treatment was very effective in stopping the death of brain cells in areas such as the cortex, hippocampus, and other areas linked with the memory and thinking. Results also manifested in the striatum, the brain area that is associated with the Parkinson’s disease in human. Hence, IGF-1 replacement reduced seizures in animals suffering from brain damage.
The researchers further suggest that IGF-1 could be used in preventing the effects of neonatal hypoxia lack of oxygen in the brain that occurs during birth. This condition could leave a baby suffering from permanent brain damage. This ability of the IGF-1 only opens doors for more possibilities, such as the reversal of the programmed cardiac cell death, following a heart attack, which can cause a victim with a heart full of dead tissues.
Keep in mind that the stroke can destroy the brain tissue, and as the cells die, the victim of a stroke is left unable to talk, walk, or think clearly. This also plays a significant role in many other neurodegenerative diseases like muscular dystrophy, Alzheimer’s disease, and multiple sclerosis (MS). For the very first time, there is a considered weapon or treatment against death at the cellular level.

Improvement of Glucose Metabolism – Similar to insulin, the IGF-1 has the same properties with the former as it can also improve the blood sugar profiles of patients with Type 2 diabetes. An increase in insulin resistance is manifested through high doses of GH, but the administration of IGF-1 can actually regulate the insulin resistance.
Researchers Bernard Beaufrere and Nelly Mauras from Jacksonville, Florida’s Nemours Children's Clinic studied several things, including the effect of IGF-1 on protein metabolism as the substance has the ability to halt protein-wasting brought about by glucocorticosteroid drugs such as prednisone, as well as its effects on glucose and insulin metabolism. The researchers divided the group of volunteers into three: IGF-1 alone, Prednisone alone, and IGF-1 plus prednisone.
The study discovered that 100 micrograms of IGF-1 per kilogram of body weight that was administered twice a day, was able to enhance the protein metabolism of the body, in the same manner as growth hormone. IGF-1 decreased the protein breakdown among the subjects who took prednisone, whereas, GH in an earlier research resulted in insulin resistance and carbohydrate intolerance when administered with prednisone did not cause diabetes-like effects. Rather, the volunteers who received IGF-1 with prednisone had a regular glucose metabolism.
The researchers said the discovery was remarkable in line with the glucocorticoids as they are known to curb insulin circulation and decrease insulin sensitivity as well. Due to this discovery and other researches, the experts now believe that IGF-1 promises treatment for protein catabolic states, such as in patients who need IV feedings after a surgery.

Helping Diabetic Patients – Two double-blinded clinical researches in 1997 also showed that recombinant IGF-1 injections could reduce the need for insulin remarkably, up to 45% in people suffering from diabetes mellitus, and who are insulin-dependent. One particular study involved eight adults aged 24 to 49, the other group was 43 children and adolescents aged 8 to 17. IGF-1 resulted in an overall lower cholesterol and triglyceride levels in the adult trial, after four days of IGF-1 treatment.
The clinical trials were only short-term, which lasted for 19 days and four weeks respectively. The insulin requirement that dropped remarkably, without serious side effects indicated that IGF-1 is a promising drug for diabetes treatment. Although it does not do away with insulin requirement, IGF-1 improved the blood sugar control, preventing further complications brought about by the disease, like blindness, peripheral nerve damage, and heart disease.

Nerve Regeneration – Furthermore, IGF-1’s potentials also include its ability in repairing the peripheral nerve tissue, which have been damaged by illness or injury. A torn nerve means that a connection to the muscle is damaged, resulting in loss of movement, along with muscle deterioration. To some extent, peripheral nerves could regenerate, but severe tears of over a few millimeters can lead to permanent injury. IGF-1, however, can repair and reconnect severely injured nerves up to six millimeters, a truly remarkable ability.
Hans-Arne Hansson, a Swedish scientist and works at the Institute of Neurobiology at the University of Goteborg, discovered that IGF-1 combined with other growth factors can stimulate a more drastic regeneration. Hansson added that IGF-1 itself, and when combined with further growth factors is likely to promote further repair and healing process.
In addition, IGF-1 has also shown significant effects on the spinal cord motor neurons, involving studies of cells in animals and culture. The motor neuron activity in the spinal cord cultures had increased by 150% up to 270%. The programmed death of cells in developing chick embryos also decreased in a relative manner. The substance also enhanced the emergence of axons of the spinal cord motor neurons in animal studies, while increasing intramuscular nerve sprouting in normal adult rats. A team of researchers at the Cephalon, Inc., West Chester, Pennsylvania said IGF-1 could be the “long-sought endogenous motor neuron sprouting factor."
In all, the implications of IGF-1 are not mind-boggling at all as it is highly capable of regenerating the spinal cord motor neurons, which is helpful in the treatment of amyotrophic lateral sclerosis (ALS), a health condition that results in the loss of cortical motor neurons and spinal cord neurons that lead to paralysis, and even death. It could likewise help in treating Charcot-Marie-Tooth syndrome, among the peripheral neuropathies conditions.
Apparently, Doctor John Wittig of the UCLA has been using the substance in preventing AIDS wasting among HIV-infected patients. The substance allows more aggressive chemotherapy in certain patients because drugs like Crisplatin and Vincristine at higher doses could lead to peripheral neuropathies.

Network of Growth Factors
Among the body’s growth factor army, the IGF-1 is now identified, isolated, and cloned through a genetic engineering technology for drug use. Doctor Eric Dupont, a growth factor researcher said, GH is equivalent to the general, while the growth factors are the foot soldiers in the army. Growth factors act as hormones, hooking onto the cell receptors, while sending biochemical signal throughout the cell’s interior. On the other hand, hormones generally send a long distance message. Most of the growth factors make the local calls.

IGF-1 as Every Bodybuilder's Dream
To date, many world-class bodybuilders are making use of IGF-1. They have reported massive muscle magnification, up by 20 pounds. Muscle Mass 2000 is an article, trumpeting IGF-1 as the most possible and most potent bodybuilding drug ever. T.C. Luoma, the author of the article said that IGF-1 is widely available in America at the moment, while sold in Venice out of the trunks of cars. In Southern California gyms alone, IGF-1 is discreetly sold in brown paper bags. There are still no supporting evidences of the bodybuilders’ claims, but the anecdotal evidence is certainly building up.
Luoma added that bodybuilders have been testifying their IGF-1 experiences, indicating 5% drop in their body fat, in only a month. The users also testified that their lean body mass increased, and their strength became more incredible. In a particular statement, it indicated that the IGF-1 is the most wonderful thing in the world. On the contrary, there are skeptics like Doctor Mauro Di Pasquale, a performance-enhancing compound expert, although there is a basis for the belief on the capacity of IGF-1 taken with HGH.
Over and above, there is a feedback mechanism between the HGH in the pituitary gland and the IGF-1 in the liver. HGH stimulates the IGF-1 release; however, there could be a rise of IGF-1 levels at a certain period in the circulation. This signals the shutdown of GH. Thus, there is a lag time throughout this process, and indication that growth hormone levels rise at night time, while IGF-1 levels rise in the daytime. Now, bodybuilders are hoping that through growth hormone and IGF-1, the effect on protein synthesis may be doubled as well.

Bottom Line
Growth hormone, most especially its army of growth factors such as the IGF-1 is widely used by bodybuilders, optimizing its bodybuilding effects, including the remarkable anti-aging reversal ability of the substance. Clinical trials have also shown IGF-1’s capability in treating various ailments like cancer and diabetes.